78446

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o-Phenylenediamine dihydrochloride

tablet, ~10 mg substrate per tablet

CAS Number:615-28-1
Linear Formula:C6H4(NH2)2·2HCl
Molecular Weight:181.06
Beilstein Registry Number:3912045
EC Number:210-418-7
MDL number:MFCD00012966
PubChem Substance ID:24887306

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Description

Applicationo-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
 Suitable as substrate for peroxidase.

Properties

product lineBioChemika
formtablet
mp258 °C (dec.)(lit.)
solubilityH2O: 1 tablet/10 mL, clear, colorless
storage temp.2-8°C

Safety

Hazard CodesT,N
Risk Statements20/21-25-36-40-43-50/53-68
Safety Statements28-36/37-45-60-61
RIDADRUN 1673 6.1/PG 3
WGK Germany3
RTECSST0175000

References

referenceBoviard, J.H. Clin. Chem. 28, 2423, (1982)
 Wolters, G., et al. J. Clin. Pathol. 29, 873, (1976)
 RegBook 1 (1), 1429:B